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Modified Apt Test

Indications
1. Assess for Vasa Previa in Late Pregnancy Bleeding
Mechanism
1. Differentiates Fetal from Maternal Blood
Technique
1. Collect bloody vaginal fluid
2. Add a small amount of tap water (Hemolyzes blood)
3. Centrifuge sample
4. Add 5 cc pink supernatant to 1 cc Sodium Hydroxide 1%
5. Read in 2 minutes (may be difficult)
  1. Pink sample indicates fetal Hemoglobin
  2. Yellow-Brown sample indicates adult Hemoglobin

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This page was written by Scott Moses, MD. This page was last revised before 5/10/08, prior to consistent tracking of revision date. This page was last published on 6/1/2008. Please send comments and questions to the editor. Contact information may be found on the page About the Family Practice Notebook. These pages are best viewed with Microsoft Internet Explorer 6.0.