II. Indication

  1. Chlamydia screening

III. Detection

  1. Methods
    1. Direct Fluorescent Antibody (DFA)
    2. Enzyme Immunoassay (EIA)
  2. Mechanism
    1. Detects both live and dead organisms
    2. Positive test may be seen in recent treatment

IV. Technique

  1. Eye Sample
    1. Topical Eye Anesthetic applied
    2. Swab lower and upper lids (inner surface)
  2. Urethral Samples (Males)
    1. Patient should not urinate within 1 hour before test
    2. Insert small Dacron swab 2-4 cm into Urethra
  3. Cervical Samples
    1. Wipe Cervix to remove excess mucus
    2. Insert Dacron swab into endocervix
    3. Rotate swab for 5 to 10 seconds
    4. Withdraw swabs without touching any vaginal surface
  4. Rectal Samples (symptomatic patients)
    1. Insert Dacron swab 3 cm into anal canal
    2. Withdraw swab
    3. Fecal contaminated samples should be redone

V. Efficacy (DFA, EIA)

  1. Sensitivity: 88-91%
  2. Specificity: 97-99%

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