Urology Book

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Urine Culture

Aka: Urine Culture
  1. Collection
    1. Sterile Methods
      1. Catheterized Urine
      2. Suprapubic Tap
    2. Clean Methods
      1. Clean Catch Urine Collection
      2. Bag Urine Collection
  2. Specimen handling
    1. Volume: 5 ml (0.2 ml minimum)
    2. First morning void is ideal specimen
    3. Deliver to lab or refrigerate within 30 minutes
  3. Interpretation: Clean Voided Specimen
    1. Normal: <10,000 organisms per ml
    2. Urinary Tract Infection
      1. Boys
        1. Organisms >10,000 per ml suggests UTI likely
      2. Girls
        1. Organisms >100,000 per ml suggests UTI
          1. Three specimens: 95% probability of UTI
          2. Two specimens: 90% probability of UTI
          3. One specimen: 80% probability of UTI
        2. Organisms >10,000 per ml needs repeat Urine Culture
  4. Interpretation: Transurethral Catheterization
    1. Normal: <1,000 organisms per ml
    2. Urinary Tract Infection
      1. Organisms >10,000 per ml suggests UTI
      2. Organisms >1,000 per ml needs repeat Urine Culture
  5. Interpretation: Suprapubic aspiration in infants
    1. Gram Negative Bacilli: any number suggests UTI (99%)
    2. Gram Positive Cocci: >1-2,000 suggests UTI (99%)
  6. References
    1. Hellerstein (1982) Pediatr Infect Dis 1:271-81 [PubMed]

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