Obstetrics Book

http://www.fpnotebook.com/

Modified Apt Test

Aka: Modified Apt Test
  1. Indications
    1. Assess for Vasa Previa in Late Pregnancy Bleeding
  2. Mechanism
    1. Differentiates Fetal from Maternal Blood
  3. Technique
    1. Collect bloody vaginal fluid
    2. Add a small amount of tap water (Hemolyzes blood)
    3. Centrifuge sample
    4. Add 5 cc pink supernatant to 1 cc Sodium Hydroxide 1%
    5. Read in 2 minutes (may be difficult)
      1. Pink sample indicates fetal Hemoglobin
      2. Yellow-Brown sample indicates adult Hemoglobin

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