III. Technique 1

  1. Fluorescein in solution or strips
    1. Fluorescein solution easily grows Pseudomonas
      1. Take care to avoid contamination
    2. Dampen strips with saline or moist Conjunctiva
  2. Fluorescein stains epithelial defects
  3. Visualize defects under cobalt blue or wood's lamp
  4. Irrigate eye after stain to avoid eye reaction to dye

IV. Technique 2

  1. Observe Corneal reflection from penlight
  2. As pt moves his eye into various positions,
    1. Epithelial disruption distorts reflection

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Ontology: Fluoresceins (C0016314)

Definition (MSH) A family of spiro(isobenzofuran-1(3H),9'-(9H)xanthen)-3-one derivatives. These are used as dyes, as indicators for various metals, and as fluorescent labels in immunoassays.
Concepts Indicator, Reagent, or Diagnostic Aid (T130) , Organic Chemical (T109)
MSH D005452
English Fluoresceins, Fluoresceins [Chemical/Ingredient]
Swedish Fluoresceiner
Czech fluoresceiny
Finnish Fluoreseiinit
Russian FLUORESTSEINY, ФЛУОРЕСЦЕИНЫ
Croatian Not Translated[Fluoresceins]
Polish Fluoresceiny pochodne
French Fluorescéines
German Fluoresceine
Italian Fluoresceine
Portuguese Fluoresceínas
Spanish Fluoresceínas