Endocrinology Book

http://www.fpnotebook.com/

Buccal Smear

Aka: Buccal Smear, Sex Chromatin Test, Barr Body
Advertisement
  1. Definitions
    1. Buccal Smear (or Sex Chromatin Test)
      1. Microscopy of cheek cell scraping
      2. Presence of barr bodies suggests 2 X-chromosomes
    2. Barr Body
      1. Inactivated X-Chromosome in each cell of a female
      2. Seen as an intracellular dark spot on microscopy
  2. History
    1. Barr Body first identified by Dr. Murray Barr in 1949
    2. Mary Lyon suggested etiology and named it Barr Body
  3. Physiology
    1. All cells in female, turn off 1 of 2 X-Chromosomes
    2. Occurs on day 16 of Embryonic development
    3. Each cell child inherits same inactivated X-Chromosome
  4. Indications
    1. Replaced by karyotype for full chromosomal mapping
    2. Previously used to evaluate abnormal Sexual Development
      1. Delayed Menarche
      2. Delayed Puberty
  5. Procedure
    1. Gently scrape inside of cheek
      1. May use wooden Tongue blade or toothpick
    2. Spread sample onto slide into drop of saline
    3. Dry slide and stain with methylene blue 0.3%
  6. Interpretation
    1. Barr Body: Crumpled dark chromosome seen in cell
      1. Suggests normal female karyotype 46XX

buccal smear biopsy procedure (C0581714)

Concepts Diagnostic Procedure (T060)
SnomedCT 31402005, 303995001
Italian Striscio orale, Striscio buccale
Dutch uitstrijkje wangslijmvlies, wangslijmvliesuitstrijkje
Japanese 頬部スミア, ﾎﾎﾌﾞｽﾐｱ, ｷｮｳﾌﾞｽﾐｱ
English Buccal smear for Barr bodies (procedure), Smear buccal, buccal smears, buccal smear, Buccal smear, Buccal smear procedure, Buccal smear procedure (procedure), Buccal smear for Barr bodies, Buccal smear for Barr bodies (procedure) [Ambiguous], buccal smear biopsy procedure
Czech Stěr z bukální sliznice
Hungarian Buccalis kenet
Spanish extendido bucal para detección de corpúsculos de Barr (concepto no activo), extendido bucal para detección de corpúsculos de Barr (procedimiento), extendido bucal para detección de corpúsculos de Barr, frotis bucal, procedimiento de extendido de material del carrillo, procedimiento de extendido yugal (procedimiento), procedimiento de extendido yugal, procedimiento de frotis yugal, Frotis bucal
Portuguese Esfregaço bucal
French Frottis buccal
German Mundabstrich
Sources
Derived from the NIH UMLS (Unified Medical Language System)


Sex Chromatin (C0036867)

Definition (MSHFRE) Dans le noyau à l'interphase, masse condensée de chromatine représentant un chromosome X inactif. Normalement, seul un chromosome X est actif dans une cellule quelque soit le nombre total de chromosomes X. Chaque chromosome X, plus un, forme la chromatine sexuelle (corpuscule de Barr). Le nombre de corpuscule de Barr présents donne une indication du nombre de chromosomes X de la cellule : Nombre de chromosomes X = nombre de corpuscules + 1.
Definition (NCI) The inactive, condensed X chromosome in a female somatic cell.
Definition (MSH) In the interphase nucleus, a condensed mass of chromatin representing an inactivated X chromosome. Each X CHROMOSOME, in excess of one, forms sex chromatin (Barr body) in the mammalian nucleus. (from King & Stansfield, A Dictionary of Genetics, 4th ed)
Definition (GO) A structure found in a female mammalian cell containing an unpaired X chromosome that has become densely heterochromatic, silenced and localized at the nuclear periphery. [GOC:hjd]
Definition (GO) A condensed mass of compacted chromatin that represents an inactivated X chromosome. [ISBN:0198506732 "Oxford Dictionary of Biochemistry and Molecular Biology"]
Concepts Cell Component (T026)
MSH D012728
SnomedCT 58348001
English Barr Bodies, Bodies, Barr, Chromatin, Sex, Chromatins, Sex, Sex Chromatin, Sex Chromatins, sex chromatin, Barr chromatin body, Barr bodies, barr bodies, chromatin sex, barr body, Barr body, Sex chromatin, Sex chromatin (substance), Barr Body
French Corpuscules de Barr, Chromatine sexuelle
Swedish Könskromatin
Czech sexchromatin
Finnish Barrin kappale
Russian X-KHROMATIN, KHROMATIN POLOVOI, Y-KHROMATIN, BARRA TEL'TSA, POLOVOI KHROMATIN, X-ХРОМАТИН, Y-ХРОМАТИН, БАРРА ТЕЛЬЦА, ПОЛОВОЙ ХРОМАТИН, ХРОМАТИН ПОЛОВОЙ
Italian Corpi di Barr, Cromatina sessuale
Latvian Dzimumhromatīns
Polish Ciałko Barra, Chromatyna płciowa
Japanese クロマチン-性, 性クロマチン, バール小体, 性染色質, 染色質-性
Spanish cromatina sexual (sustancia), cromatina sexual, cuerpo de cromatina de Barr, Corpusculos de Barr, Cromatina Sexual
German Chromatin, Geschlechts-, Geschlechtschromatin, Barr-Körperchen
Dutch Barr-lichaampjes, Chromatine, sekse-, Seksechromatine
Portuguese Corpos de Barr, Cromatina Sexual
Sources
Derived from the NIH UMLS (Unified Medical Language System)


Navigation Tree