II. Technique: Staining

  1. Staining
    1. Avoid water contamination
    2. Romanowsky variants based on methylene blue and eosin
      1. Wright Stain (Sodium Bicarbonate)
      2. May-Grunwald-Giemsa Stain (acid bichromate)
  2. Slide evaluation
    1. Evaluate for adequate sample under 10x power
    2. Cell exam under oil immersion lens at 50x or higher

IV. References

  1. Lee (1999) Wintrobe's Hematology, Lippincott, p. 23-25

Images: Related links to external sites (from Google)

Ontology: Peripheral blood smear interpretation (C0430246)

Concepts Laboratory Procedure (T059)
SnomedCT 14768001, 143096002, 165695005
English Blood film examination, Peripheral blood smear interpretation, Peripheral blood film examination, Peripheral blood smear interpretation (procedure), Blood film
Spanish Blood film, interpretación de extendido de sangre periférica (procedimiento), interpretación de extendido de sangre periférica, interpretación de frotis de sangre periférica