Hematology and Oncology Book

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Peripheral SmearAka: Peripheral Blood Film, Blood Film

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  1. See Also
    1. Complete Blood Count
    2. Bone Marrow Exam
  2. Technique: Staining
    1. Staining
      1. Avoid water contamination
      2. Romanowsky variants based on methylene blue and eosin
        1. Wright Stain (Sodium Bicarbonate)
        2. May-Grunwald-Giemsa Stain (acid bichromate)
    2. Slide evaluation
      1. Evaluate for adequate sample under 10x power
      2. Cell exam under oil immersion lens at 50x or higher
  3. Examination
    1. See Erythrocyte Morphology
    2. See Leukocyte Morphology
    3. See Platelet Morphology
  4. References
    1. Lee (1999) Wintrobe's Hematology, Lippincott, p. 23-25

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