II. Technique: Staining

  1. Staining
    1. Avoid water contamination
    2. Romanowsky variants based on methylene blue and eosin
      1. Wright Stain (Sodium Bicarbonate)
      2. May-Grunwald-Giemsa Stain (acid bichromate)
  2. Slide evaluation
    1. Evaluate for adequate sample under 10x power
    2. Cell exam under oil immersion lens at 50x or higher

IV. References

  1. Lee (1999) Wintrobe's Hematology, Lippincott, p. 23-25

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Ontology: Peripheral blood smear interpretation (C0430246)

Concepts Laboratory Procedure (T059)
SnomedCT 165695005, 143096002, 14768001
CPT 85060
English Blood film examination, Interpretation of peripheral blood smear, Blood film, Peripheral blood smear interpretation, Peripheral blood film examination, Peripheral blood smear interpretation (procedure)
Spanish interpretación de extendido de sangre periférica (procedimiento), interpretación de extendido de sangre periférica, interpretación de frotis de sangre periférica