Hematology and Oncology Book

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CoombsAka: Coombs Reagent

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  1. Coombs Reagent
    1. Anti-Human Globulin Antibody
    2. Binds to human IgG Antibody and C3 Complement
    3. Produced in rabbits by Immunization
  2. Direct Coombs
    1. Question
      1. Is IgG or complement bound to Red Blood Cell?
    2. Technique
      1. Start with patient's Red Blood Cells
      2. Add Anti-human globulin antibodies (Coombs reagent)
    3. Direct Coombs Positive
      1. Agglutination indicates Antibody coated RBCs
    4. Example of use
      1. Are maternal antibodies bound to fetal RBCs?
  3. Indirect Coombs
    1. Question
      1. Is Antibody against Red Blood Cell present in serum?
    2. Technique
      1. Start with patient's serum
      2. Add Anti-human globulin antibodies (Coombs reagent)
      3. Add Indicator Red Blood Cells with known antigens
    3. Indirect Coombs Positive
      1. Agglutination indicates Antibody to RBC in serum
      2. Antibody titer can be obtained by serial dilution
    4. Example of use
      1. Are antibodies to fetal RBC present in maternal serum

Coombs' Test (C0009961)

Definition (MSH)Hemagglutination test in which Coombs' reagent (antiglobulin, or anti-human globulin rabbit immune serum) is added to detect incomplete (non-agglutinating, univalent, blocking) antibodies coating erythrocytes. The direct test is applied to red cells which have been coated with antibody in vivo (e.g., in hemolytic disease of newborn, autoimmune hemolytic anemia, and transfusion reactions). The indirect test is applied to serum to detect the presence of antibody (e.g., in detection of incompatibility in cross-matching tests, detection and identification of irregular antibodies, and in detection of antibodies not identifiable by other means).
Definition (NCI)Hemagglutination test in which Coombs' reagent (antiglobulin, or anti-human globulin rabbit immune serum) is added to detect incomplete (non-agglutinating, univalent, blocking) antibodies coating erythrocytes. The direct test is applied to red cells which have been coated with antibody in vivo (e.g., in hemolytic disease of newborn, autoimmune hemolytic anemia, and transfusion reactions). The indirect test is applied to serum to detect the presence of antibody (e.g., in detection of incompatibility in cross-matching tests, detection and identification of irregular antibodies, and in detection of antibodies not identifiable by other means). (MeSH)
ConceptsLaboratory Procedure (T059)
EnglishAnti Human Globulin Consumption Test, Antiglobulin Consumption Test, Antiglobulin Consumption Tests, Antiglobulin Test, Antiglobulin Tests, ANTIHUMAN GLOBULIN CONSUMPTION TEST, Coomb Test, Coomb's Test, Coombs, Coombs Test, Coombs' Test
CreditsDerived from the NIH UMLS (Unified Medical Language System)



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